MODIFICATION OF OPTICAL SPECTRA AND CYTOSTATIC ACTIVITY OF DOXORUBICIN BY IONIZING RADIATION

Abstract

Introduction. The regularities of the effect of prior electron irradiation of saline solution on the optical and cytotoxic properties of saline solution and doxorubicin dissolved in it are studied in order to increase their cytostatic efficiency.

Methods. The energy of the irradiation electrons was equal to 1 MeV, the absorbed dose was within (2...80) kGy. It has been reported that the amount of absorbed radiation dose affects the optical characteristics of saline in the UV, visible, and IR regions. Changes in the extinction spectra of the physiological solution depended on both the absorbed dose and the wavelength of the light.

Results. It was established that radiation-induced changes in the extinction properties and cytostatic activity of the irradiated physiological solution last 2-4 months, depending on the amount of the absorbed dose. It is shown that the fluorescence intensity of physiological solution caused by excitation at a wavelength of 260 nm reaches the highest value at the value of the absorbed radiation dose (10...40) kGy. It is shown that the position of the bands of the fluorescence spectra depends on the amount of the absorbed dose. It was established that despite the rearrangement of the fluorescence center, the number of spectral bands due to radiation exposure remains constant.

Conclusions. It was shown that pre-irradiation of the FR before dissolving doxorubicin in it leads to an increase in its cytostatic activity. This effect is most pronounced at relatively low concentrations of doxorubicin in the solution. The minimum dose of radiation absorbed by the physiological solution, at which changes in the spectra of the doxorubicin solution are recorded, is 4 kGy.

References

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Zabolotny, M.A., Aslamova, L.I., Dovbeshko, G.I., Hnatyuk, O.P., Neimash, V.B., Povarchuk, V.Yu., Orel, V.E., Kolesnyk, D.L., Kirkilevska, L.M., & Solyanyk, G.I. (2022). The effect of irradiation of physiological solution with high-energy electrons on the physicochemical properties and cytostatic activity of doxorubicin. Nuclear Physics and Atomic Energy, 23(2), 131–139 [in Ukrainian]. https://doi.org/10.15407/jnpae2022.02.131

Zabolotnyy, M.A., Aslamova, L.I., Dovbeshko, G.I., Hnatiuk, O.P., Solyanyk, G.I., Dankevich, V.P., Leonov, D.S., Barabash, M.Yu., & Chernyak, V.A. (2024). Effect of high-energy electron irradiation of saline solution on its optical characteristics and cytotoxicity/cytodynamic activity. Nanosystems, Nanomaterials, Nanotechnologies, 22(2), 1001–1008 [in Ukrainian]. https://doi.org/10.15407/nnn.22.02.481