ANTIBACTERIAL PROPERTIES OF HYDROGELS BASED ON POLYACRYLAMIDE AND POLYHEXAMETHYLENEGUANIDINE HYDROCHLORIDE

Abstract

Introduction. The research is devoted to developing biomedical polymer materials that have antibacterial properties.

Methods. Two hydrophilic synthetic polymers – covalently cross-linked polyacrylamide and poly hexamethylene guanidine hydrochloride – were synthesized by radical polymerization and polycondensation, respectively.

Results. As a result of limited swelling, cross-linked polyacrylamide in aqueous solution forms hydrogels with good diffusion and sorption properties, the degree of swelling is 1370 %. The bactericidal properties of PGMHC were evaluated by determining the minimum inhibitory concentration of strains Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella quasipneumoniae bacteria and showed the high activity of the polymer in aqueous solution at concentrations less than 175 μg/ml. As a result of the sorption of PGMHC from solutions of different concentrations onto the surface of the hydrogel, samples with different contents of the active substance in the sample were obtained. Samples of PAA hydrogels from PGMHC were tested by the disk diffusion method against the strains of the specified bacteria, and their antibacterial properties were shown.

Conclusions. Thus, PAA hydrogels with poly hexamethylene guanidine hydrochloride adsorbed on their surface, possessing low toxicity, biological destruction, and non-aggressiveness to other materials and biological tissues, due to their antibacterial properties can be used as wound dressings that prevent infectious diseases.

References

Nadtoka, O., Kutsevol, N., Linnik, O., & Nikiforov M. (2019). Nanocomposite Hydrogels Containing Silver Nanoparticles as Materials for Wound Dressings. Springer Proceedings in Physics, 222, 375–387. https://doi.org/10.1007/978-3-030-17755-3_24

Nadtoka, O., Virych, P., & Kutsevol, N. (2021). Synthesis and absorption properties of hybrid polyacrylamide hydrogels. Molecular Crystals and Liquid Crystals, 719(1), 84–93. https://doi.org/10.1080/15421406.2020.1862464

Nizhnik, T.Yu., Baranova, A.I., Rokitska, O.V., Nizhnik, V.V. (2004). Complex formation of copper ions with polyhexamethyleneguanidine in an aqueous environment. Zurnal Hromatograficnogo tovaristva Journal of the Chromatographic Society, ІV(2), 2, 16–25 [in Ukrainian]. https://doi.org/10.15407/zht

Salmanov, A.G. (2017). Resistance to antibiotics and biocides. International Journal of Antibiotics and Probiotics, 1(2), 92–125 [in Ukrainian]. https://doi.org/10.31405/ijap.1-2.17.07

Walczak, M., Richert, A., & Burkowska-But, A. (2014). The effect of polyhexamethylene guanidine hydrochloride (PHMG) derivatives introduced into polylactide (PLA) on the activity of bacterial enzymes. Journal of Industrial Microbiology and Biotechnology, 41(11), 1719–1724. https://doi.org/10.1007/s10295-014-1505-5